Professore Associato 
Settore scientifico disciplinare di riferimento  (BIO/10)
Ateneo Università degli Studi di ROMA "La Sapienza" 
Struttura di afferenza Dipartimento di SCIENZE E BIOTECNOLOGIE MEDICO-CHIRURGICHE 
Recapiti Elenco recapiti telefonici
Fax 0773 1757254

Orari di ricevimento

giovedì ore 10-12 (Latina) venerdì ore 14-15 (Roma)


Given name Daniela
Surname De Biase
Present Work Address Department. of Medico-Surgical Sciences and Biotechnologies
Sapienza University of Rome
Corso della Repubblica, 79 - 04100 Latina, Italy

Phone: +39-0773-1757212 (office) / +39-0773-1757230 (lab)
Fax: +39-0773-1757254
Nationality Italian
Date of birth 14 June 1964
Gender Female

Occupation field BIO10 Biochemistry (Primary); Molecular Biology – Microbiology – Biotechnology
Work experience
June 2010 – to date Associate Professor of Biochemistry
Main activities Teaching and Research
Employer Dpt. of Medico-Surgical Sciences and Biotechnologies - Sapienza University of Rome

July 2010
Position held Visiting Professor
Main activities Research
Employer Laboratorie de Biologie and Pharmacologie Appliquée (LBPA), CNRS-École Normale Supérieure (ENS) de Cachan -France.

March 2001 – May 2010
Occupation Associate Professor of Biochemistry
Main activities Teaching and Research
Employer Dpt. of Biochemical Sciences “A. Rossi Fanelli” - Sapienza University of Rome

November 1991 – February 2001
Occupation Assistant Professor (Researcher) of Biochemistry
Main activities Teaching and Research
Employer Dpt. of Biochemical Sciences “A. Rossi Fanelli” - University of Rome “La Sapienza”
Education and training September-October 1995
Qualification Visiting scientist: stopped-flow kinetics
Organization Biochemistry Dpt, University of Wales, Cardiff, Wales (UK).
August 1994
Qualification Visiting scientist: screening of lambda phage libraries
Organization Institut fur Planzengenetik und Kulturplanzenforschung (IPK) Gatersleben, Germany.

September 1991
Qualification Visiting scientist: crystallization of proteins
Organization Biozentrum, Basel (Switzerland)

March 1991
Title of qualification awarded Ph.D. Biochemistry
Thesis title “Chemistry of the mechanism-based inactivation of pyridoxal phosphate-dependent enzymes”
Organization University of Wales, College of Cardiff, Cardiff (UK)

March 1988 – March 1991
Training Ph.D student: Mechanism of inactivation of mammalian GABA-transaminase and ornithine transaminase by suicide inhibitors (recipient of funding from European
Project,Human Capital and Mobility)
Organization University of Wales, College of Cardiff, Cardiff (UK)

December 1987
Title of qualification awarded Master Degree In Biological Sciences (summa cum laude)
Thesis title “Modern methodologies for the determination of the primary structure of proteins”
Organization University of Rome “La Sapienza”

September 1986 – February 1988
Training Undergraduate student: Bioactive peptides from amphibian skin - gas phase Edman sequencing.
Organization Dpt. of Biochemical Sciences “A. Rossi Fanelli” - University of Rome “La Sapienza”,

February 1986 – August 1986
Training Undergraduate student: epidemiological study on HIV infection and cell culturing (lymphocytes)
Organization Istituto Superiore di Sanità (ISS) - Rome

Personal skills and competences General fields:
Biochemistry, Molecular Biology, Microbiology, Biotechnology
Specific fields:
Acid resistance and stress response in enteric bacteria.
Structure-function relationships in PLP-dependent enzymes in particular linked to glutamate and GABA metabolism. Enzyme kinetics.
Protein-DNA interactions

Mother tongue(s) Italian
Other language(s) English
Teaching (16 CFU/year)
- 2012-to date: Biochemistry I and II, International Medical School (taught in english), University of Rome “La Sapienza” (5 CFU)
- 2000-to date: Biochemistry I, Medical School (Latina), University of Rome “La Sapienza” (5 CFU)
- 2001-2017: Biochemistry, First Cycle Degree in Speech Therapy, University of Rome “La Sapienza (2 CFU)
- 2008-to date: Biochemistry, First Cycle Degree in Midwifery, University of Rome “La Sapienza” (2 CFU)
- 2010-to date: Biochemistry, First Cycle Degree in Physiotherapy, University of Rome “La Sapienza” (2 CFU)
Institutional activities:
- 2012 –to date: Faculty Member in the Ph.D. Course “Life Sciences” in University of Rome “La Sapienza”
- 2010-to date: Member of the technical-pedagogical Board, Medical School (Latina), University of Rome “La Sapienza”
- 2000-to date: Semester Coordinator, Medical School (Latina), University of Rome “La Sapienza”
- 2017- : Coordinator of Mobility Activities (CAM) - Faculty of Pharmacy and Medicine (Medical Area)
- 2017- : Member of Traineeship Committee - Faculty of Pharmacy and Medicine (Medical Area)
- Sapienza-École Normale Supérieure de Cachan (France) academic year 2013-2014;
- Sapienza-University of Eastern Finland (Kuopio, Finland) Erasmus+ 2014-2020 (to date 3 students exchanged).
Students scientific training:
1992-to date: Daniela De Biase has trained in research
- >30 Master students (Biology, Biotechnology and Chemistry and Technologies of Drugs) with experimental thesis work lasting 18 months/student.
- supervised/co-supervised 9 PhD students (3-years course duration).
- Tutor of Andreu Coello Pelegrin from Universitat Autonoma de Barcelona, Spain (EURACCIÓ 2014, Leonardo da Vinci Programme; 6 months: May-Oct 2014)
- Tutor of undergraduate student Chayan Kothari from Indian Institute of Technology (BHU), Varanasi (summer traineeship of 10 weeks: May-July 2014).
Research lines
Microbiology-Molecular Biology: working on the transcriptional regulation and the biochemistry of the Gad system, the major acid resistance (AR) system in Escherichia coli (commensal and pathogenic) and many other enteric bacteria.
Several interconnections between different AR systems and other physiological processes in E. coli and other enteric bacteria are currently under investigation.
Presently working on new molecules of natural and synthetic origin as potential antibiotics.
Biochemistry: Expert on PLP-dependent enzymes, in particular glutamate decarboxylase (GAD) and GABA-transaminase (GABA-T) of mammalian and bacterial origin
Biotechnology: Engineering GAD for biotech applications.
Ongoing International Collaborations (in the last 10 years):
2009-present: Dr. Sylvie Rimsky, College de France, Paris (France).
2010-present: Dr. Alessandra Occhialini. CPBS, University of Montpellier (France) .
2013-present: Dr Alex Khomutov, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow (Russia).
2013-present: Prof. Jouko Vepsalainen, School of Pharmacy, University of Eastern Finland, Kuopio, (Finland).
2015-present: Prof. Jean-Marc Ghigo, Institute Pasteur, Paris (France).
2015-present: Prof. John Blanchard (Albert Einstein, College of Medicine, New York (USA)
2017-present: Dr. Tim Clausen, Institute of Molecular Pathology, Vienna (Austria).
Affiliation to Scientific Societies
1991 - to date: Società Italiana di Biochimica e Biologia Molecolare (SIB)
2009 - to date: American Society for Microbiology (ASM)
2017 - to date: Microbiology Society (UK).
2016- to date: Board Member of the Microbial Physiology Section of the European Federation of Biotechnology (EFB),
Professional Links
Google Scholar:
Reviewing activities Daniela De Biase acts as reviewer for the following international journals: Molecular Microbiology, Infection and Immunity, Virulence, Biochemistry, Microbial Cell Factories, FEMS Microbiology Letters, Microbiology, Archives in Microbiology, FEBS Letters, BMC Microbiology, Biotechnology and Bioengeneering, International Journal of Molecular Sciences, FEBS Journal, Molecular Biosystems, Enzyme and Microbial Technology, Genes.
Certified by Publons:
She evaluates projects for BBSRC (UK), ANR (France) and Università Italo-Francese (UIF).
In the Editorial Board of:
1) Frontiers in Microbiology(Associate Editor for Microbial Physiology and metabolism)
2) AIMS Microbiology
Scientific Publications (max. 30)
1. Freddi L, Damiano MA, Chaloin L, Pennacchietti E, Al Dahouk S, Köhler S, Daniela De Biase D, Occhialini A. (2017) The glutaminase-dependent system confers extreme acid resistance to new species and atypical strains of Brucella.
Frontiers in Microbiology. 8:2236. doi: 10.3389/fmicb.2017.02236
2. Tramonti A, De Santis F, Pennacchietti E, De Biase D.* (2017) The yhiM gene codes for an inner membrane protein involved in GABA export in Escherichia coli.
AIMS Microbiology. 3(1):71-87. doi: 10.3934/microbiol.2017.1.71.
3. Giovannercole F, Mérigoux C, Zamparelli C, Verzili D, Grassini G, Buckle M, Vachette P, De Biase D.* (2017) On the effect of alkaline pH and cofactor availability in the conformational and oligomeric state of Escherichia coli glutamate decarboxylase.
Protein Eng Des Sel. 30(3):235-244. doi: 10.1093/protein/gzw076.
4. Khomutov МA, Formanovsky АА, Mikhura IV, Vepsalainen J, Kochetkov SN, De Biase D**, Khomutov АR (2016) Convenient synthesis of phosphinic analogues of -aminobutyric and glutamic acids.
Russ. J. Bioorganic Chem. 42(6):672-676. doi: 10.1134/S1068162016060042.
5. Pinto A, Tamborini L, Pennacchietti E, Coluccia A, Silvestri R, Cullia G, De Micheli C, Conti P, De Biase D. (2016) Bicyclic γ-amino acids as inhibitors of γ-aminobutyrate aminotransferase.
J Enzyme Inhib Med Chem. 31(2):295-301. doi: 10.3109/14756366.2015.1021251.
6. De Biase D and Lund P. (2015) The Escherichia coli Acid Stress Response and Its Significance for Pathogenesis.
Adv Appl Microbiol. 92:49-88. doi: 10.1016/bs.aambs.2015.03.002.
7. Grassini G, Pennacchietti E, Cappadocio F, Occhialini A, De Biase D.* (2015) Biochemical and spectroscopic properties of Brucella microti glutamate decarboxylase, a key component of the glutamate-dependent acid resistance system.
FEBS Open Bio 5:209-18. doi: 10.1016/j.fob.2015.03.006.
8. Damiano MA, Bastianelli D, Al Dahouk S, Köhler S, Cloeckaert A, De Biase D**, Occhialini A (2015) Glutamate decarboxylase-dependent acid resistance in Brucella spp.: distribution and contribution to fitness under extreme acid conditions.
Appl Environ Microbiol 81(2):578-86. doi: 10.1128/AEM.02928-14.
9. Kass I, Hoke DE, Costa MGS. Reboul CF, Porebski BT, Cowieson NP, Leh H, Pennacchietti E, McCoey J, Kleifeld O, Borri Voltattorni C, Langley D, Roome B, Mackay IR, Christ D, Perahia D, Buckle M, Paiardini A, De Biase D, Buckle AM (2014) Cofactor-dependent conformational heterogeneity of GAD65 and its role in autoimmunity and neurotransmitter homeostasis.
Proc Natl Acad Sci U S A. 111(25):E2524-9. doi: 10.1073/pnas.1403182111.
10. Lund P, Tramonti A, De Biase D.* (2014) Coping with low pH: molecular strategies in neutralophilic bacteria.
FEMS Microbiol Rev. 38(6):1091-125. doi: 10.1111/1574-6976.12076.
11. Di Pietro M, De Santis F, De Biase D, Sessa R (2013) The elusive but pathogenic peptidoglycan of Chlamydiae.
Eur J Inflamm 11(1):257-60. doi: 10.1177/1721727X1301100126.
12. Occhialini A, de Bagüés MP, Saadeh B, Bastianelli D, Hanna N, De Biase D, Köhler S (2012) The glutamic acid decarboxylase system of the new species Brucella microti contributes to its acid resistance and to oral infection of mice.
J. Infect. Dis. 206(9):1424-32. doi: 10.1093/infdis/jis522.
13. De Biase D*, Pennacchietti E. (2012) Glutamate decarboxylase-dependent acid resistance in orally acquired bacteria: function, distribution and biomedical implications of the gadBC operon.
Mol. Microbiol 86(4):770-86. doi: 10.1111/mmi.12020.
14. Saliola M, Tramonti A, Lanini C, Cialfi S, De Biase D, Falcone C (2012) Intracellular NADPH levels affect the oligomeric state of the glucose 6-phosphate dehydrogenase in the yeast Kluyveromyces lactis.
Eukaryotic Cell. 11(12):1503-11. doi: 10.1128/EC.00211-12.
15. Di Pietro M, Tramonti A, De Santis F, De Biase D, Schiavoni G, Filardo S, Zagaglia C, Sessa R (2012) Analysis of gene expression in penicillin G induced persistence of Chlamydia pneumoniae.
J Biol Regul Homeost Agents. 26(2):277-84.
16. Doucette GJ, Mikulski CM, King KL, Roth PB, Wang Z, Leandro LF, Degrasse SL, White KD, De Biase D, Gillett RM, Rolland RM (2012) Endangered North Atlantic right whales (Eubalaena glacialis) experience repeated, concurrent exposure to multiple environmental neurotoxins produced by marine algae.
Environ.Res.112:67-76. doi: 10.1016/j.envres.2011.09.010.
17. Yu Z, Bekker M, Tramonti A, Cook GM, van Ulsen P, Scheffers DJ, de Mattos JT, De Biase D, Luirink J (2011) Activators of the glutamate-dependent acid resistance system alleviate deleterious effects of YidC depletion in Escherichia coli.
J Bacteriol. 193(6):1308-16. doi: 10.1128/JB.01209-10.
18. Pennacchietti E, Lammens TM, Capitani G, Franssen MC, John RA, Bossa F, De Biase D* (2009) Mutation of His465 alters the pH-dependent spectroscopic properties of Escherichia coli glutamate decarboxylase and broadens the range of its activity toward more alkaline pH.
J Biol Chem. 284(46):31587-96. doi: 10.1074/jbc.M109.049577.
19. Lammens TM, De Biase D, Franssen MCR, Scott EL, Sanders JPM (2009) The application of glutamic acid alpha-decarboxylase for the valorization of glutamic acid.
Green Chemistry 11:1562-67. doi: 10.1039/B913741F.
20. Tramonti A, De Canio M, De Biase D* (2008) GadX/GadW-dependent regulation of the Escherichia coli acid fitness island: transcriptional control at the gadY-gadW divergent promoters and identification of four novel 42 bp GadX/GadW-specific binding sites.
Mol Microbiol. 70(4):965-82. doi: 10.1111/j.1365-2958.2008.06458.x.
21. Fiorini F, Stefanini S, Valenti P, Chiancone E, De Biase D. (2008) Transcription of the Listeria monocytogenes fri gene is growth-phase dependent and is repressed directly by Fur, the ferric uptake regulator.
Gene 410(1):113-121. doi: 10.1016/j.gene.2007.12.007.
Books Chapters (Last 5 years)
1. Giovannercole F, Pennacchietti E, De Biase D.* (2017) Glutamate decarboxylase in Bacteria.
In: Handbook of Microbial Metabolism of Amino acids (ed. J.P.F. D’Mello) CAB International (In press).
2. Pennacchietti E, Giovannercole F, De Biase D.* (2016) Acid survival mechanisms in neutralophilic bacteria.
In: Stress and Environmental Control of Gene Expression and Adaptation in Bacteria (ed. F. J. de Bruijn) John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781119004813.ch89.
3. Bonamore A, Macone A, Kroutil W, Seisser B, Lammens TM, De Biase D, Franssen MCR. Scott EL, Sanders JPM, Miyamoto K, Kourist R, Yoshida S, Ohta H, Tian J, Li H, Gao W-Y, Shanmuganathan S, Natalia D, Greiner L, de María PD, He Y-H, Guan Z, Cai J-F (2012) C–C Bond Formation and Decarboxylation.
In: Practical Methods for Biocatalysis and Biotransformations 2. (ed. J Whittall and P.W. Sutton). p. 263-295, Chichester:John Wiley & Sons, Ltd.

Delivered seminars/talks (last 5 years)
1. 2016: “Catalytic mechanism and structural organization of Escherichia coli Glutamate Decarboxylase” V Congress of the Russian Biochemical Society- V CIS Congress on Physiology, Sochi – Dagomys, Russia October 4–9, 2016 (invited speaker).
2. 2015: “Acid stress resistance of gut bacteria: characterization of the GadX regulon in Escherichia coli” Collège de France – Paris (seminar upon invitation; November 30, 2015).
3. 2015: “Role of the glutamate decarboxylase (GAD)-dependent system of Brucella spp. in the resistance to extreme acid stress”. Microbial Stress: From Molecules to Systems. Sitges, Spain 12-15 November, 2015 (selected speaker).
4. 2015: “Glutamate decarboxylase from neutralophilic bacteria: from biochemistry, to physiology and biotechnology” The Francis Crick Institute - Mill Hill Laboratory. London, UK; May 7, 2015 (seminar upon invitation)
5. 2013 "Some like it acid: how Escherichia coli glutamate decarboxylase controls its intracellular activity in response to acid stress". FEBS Congress 2013 "Mechanisms in Biology", St. Petersburg (Russia); July 6-11, 2013 (invited speaker)
6. 2013: “The GAD system of Escherichia coli: biochemical, structural and regulatory aspects” CPBS-CNRS Montpellier, France; April 26. 2013 (seminar upon invitation)
7. 2013: “Beyond acid resistance: identification of novel targets of the regulators of the gad system in Escherichia coli” University of Birmingham, UK; March 13, 2013 (seminar upon invitation)