Professore Associato 
Settore scientifico disciplinare di riferimento  (BIO/17)
Ateneo Università degli Studi di ROMA "La Sapienza" 
Recapiti Elenco recapiti telefonici

Orari di ricevimento

martedi 10-13; giovedi 14-17, previo appuntamento


Place & Date of Birth Rome, 29.12.1956
Nationality Italian
Education Degree in Biology, with honours, Sapienza Univ. of Rome, 1979,
Maturità Classica, Liceo T. Mamiani, Rome, 1974
Address Dept. of Anatomy, Histology, Forensis Medicine and Orthopedics, Unit of Histology and Med. Embryology, Sapienza Univ. of Rome, Via A. Scarpa 14, 00161 Rome, Italy.
Tel. +39 (6) 4976 6755 Fax +39 (6) 4462854
Present Position
Associate Professor in Histology, Medical School, Sapienza Univ. of Rome.
Professor of: Histology and Embryology at the Dental School; Histology and Embryology at the International Medical School, CLM F; Histology at the Biotechnological Science School (1st level); Cell Biotechnology at the Biomedical Technology Science School (2nd level); Histology at the Nursing School (1st level). Member of the academic committee of the PhD program in “Morphogenesis and Tissue Engineering”. Member of the Interuniversity Institute of Myology. Member of the Centre of Excellence in “Biology and Molecular Medicine” (BEMM).
Head of the Laboratory of muscle histogenesis and remodelling, Unit of Histology and Med. Embryology.
Previous professional experience
2008 Visiting scientist, Groupe Miologie, UMR S 787 Inserm Université Paris 6 PMC, Paris.
1990-2001 Research Associate, Dept. of Histology, Sapienza Univ. of Rome;
1993 Visiting scientist (NCI) Cell Biol. UT Southwestern at Dallas, USA;
1986-1990 Post_doctoral Research Fellow, Inst. of Histology, Sapienza Univ. of Rome;
1984-1986 Post_doctoral Research Fellow, Cell Biology, Cornell Univ. Med. College, New York, USA;
1982-1984 Post_doctoral Research Fellow, Inst. of Histology, Sapienza Univ. of Rome;
1979-1982 Instructor, Inst. of Histology, Sapienza Univ. of Rome.
Fields of interests
Signalling pathways regulating skeletal and cardiac muscle differentiation, histogenesis and remodelling. Signalling pathways regulating myogenic differentiation in rhabdomyosarcoma cells.
Memberships of Journal Editorial Boards
2011-present The Scientific World Journal
Meetings and Workshops
Dr. Bouche has been presenting her work at meetings and workshops organized by many scientific societies among which: EMBO Workshops on Myogenesis, American Society of Cell Biology, American Society of Biological Chemists, Biophysical Society, American Society of Anatomists, Keystone Symposia on Muscle Development, British Society for Experimental Biology, ABCD, FISV, Gordon Conferences on Myogenesis.

Selected Publications
• Marrocco V, Fiore P, Benedetti A, Pisu S, Rizzuto E, Musarò A, Madaro L, Lozanoska-Ochser B, Bouché M. Pharmacological inhibition of PKCθ counteracts muscle disease in a mouse model of Duchenne Muscular Dystrophy. EBioMedicine, 16: 150-161, 2017
• Klionsky D.J., K. Abdelmohsen,… J. Botti, G. Bou, M. Bouché, M. Bouchecareilh, M. Boucher,…. T. Zoladek, W. Zong, A. Zorzano, S.M. Zughaier. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy, 12: 1-222, 2016
• Smeriglio P., S. Alonso-Martin, S. Masciarelli, L. Madaro, I. Iosue, V. Marrocco, F. Relaix, F. Fazi, G. Marazzi, D. A. Sassoon, M. Bouché. Phosphotyrosine phosphatase inhibitor bisperoxovanadium endows myogenic cells with enhanced muscle stem cell functions via epigenetic modulation of Sca-1 and Pw1 promoters. Faseb J. 30:1404-1415, 2016 ; published ahead of print December 15, 2015
• Faggi F., N. Chiarelli, M. Colombi, S. Mitola, R. Ronca, L. Madaro, M. Bouche, P.L. Poliani, M. Vezzoli, F. Longhena, E. Monti, B. Salani, D. Maggi, C. Keller, A. Fanzani. Cavin-1 and Caveolin-1 are both required to support cell proliferation, migration and anchorage-independent cell growth in rhabdomyosarcoma. Lab. Invest. advance online publication 30 March 2015
• Bouche M., L. Madaro. Invited Commentary on "Enrichment and Characterization of Two Subgroups of Committed Osteogenic Cells in the Mouse Endosteal Bone Marrow with Expression Levels of CD24”. J Bone Marrow Res 2: 145, 2014.
• Marrocco V., P. Fiore, L. Madaro, A. Crupi , B. Lozanoska-Ochser, M. Bouché. Targeting PKCθ in skeletal muscle and muscle diseases: good or bad? Biochem Soc Trans. 42:1550-5, 2014.
• Bouché M., P. Muñoz-Cánoves, F. Rossi, and D. Coletti . Inflammation in Muscle Repair, Aging, and Myopathies. BioMed Res. Int. 2014: 821950. Epub 2014 Aug 4
• Madaro L. and M. Bouché. From Innate to Adaptive Immune Response in Muscular Dystrophies and Skeletal Muscle Regeneration: The Role of Lymphocytes. BioMed Res. Int. 2014:438675. Epub 2014 Jun 16
• Camerino GM., M. Bouchè, M. De Bellis, M. Cannone, A. Liantonio, K. Musaraj, R. Romano, P. Smeriglio, L. Madaro, A. Giustino, A. De Luca, JF. Desaphy, D. Conte Camerino, S. Pierno. Protein kinase C theta (PKCθ) modulates the ClC-1 chloride channel activity and skeletal muscle phenotype: a biophysical and gene expression study in mouse models lacking the PKCθ. Pflugers Arch - Eur J Physiol 2014 Mar 20 [Epub ahead of print]
• Madaro L, F. Antonangeli, A. Favia, B. Esposito, F. Biamonte, M. Bouché, E. Ziparo, G. Sica, A. Filippini, A. D'Alessio. Knock down of caveolin-1 affects morphological and functional hallmarks of human endothelial cells. J Cell Biochem. 114: 1843-51, 2013
• Madaro L., V. Marrocco, S. Carnio, Sandri M. and M. Bouché. Intracellular signaling in ER stress-induced autophagy in skeletal muscle cells. Faseb J. 27:1990-2000, 2013. [Epub 2013 Feb 6]
• Madaro L., A. Pelle, C. Nicoletti, A. Crupi, V. Marrocco, G. Bossi, S. Soddu and M. Bouché. PKC theta ablation improves healing in a mouse model of muscular dystrophy. PLoSOne7: e31515. Epub 2012 Feb 14
• Verga Falzacappa C., C. Mangialardo, L. Madaro, S. Raffa, D. Ranieri, A. Stigliano, E. Brunetti, MR. Torrisi, M. Bouchè, V. Toscano and S. Misiti. Thyroid hormone T3 prevents STZ induced diabetes in mouse PLoSOne 6:e19839. Epub 2011 May 27
• Quattrocelli M, G Palazzolo, I Agnolin, S Martino, M Bouché, L Anastasia, M. Sampaolesi. Synthetic sulfonyl-hydrazone-1 positively regulates cardiomyogenic microRNA expression and cardiomyocyte differentiation of induced pluripotent stem cells. J. Cell. Biochem, 112: 2006-14, 2011
• Madaro L., V. Marrocco, P. Fiore, P.Aulino, P. Smeriglio, S. Adamo, M. Molinaro and M. Bouché. Protein kinase C theta signaling is required for myoblast fusion by regulating the expression of caveolin-3 and β1D integrin upstream Focal Adhesion Kinase. Mol. Biol. Cell, 22: 1409-1419, 2011. [Epub 2011, Feb 23].
• Paoletti R., A. Notte, A. Maffei, E. Stanganello, G. Cifelli, G. Selvetella Molinaro M., Lembo G. and Bouché M. Protein kinase C theta is required for cardiomyocytes survival and cardiac remodelling. Cell Death and Disease, 1: e45, Epub 2010 May 27
• Madaro L., Smeriglio P., Molinaro M. and Bouché M.. Unilateral hindlimb immobilization: a simple model of atrophy in mice. Basic and Applied Myology, 18: 149-153, 2008
• Castaldi L., Serra C., Moretti F., Messina G., Paoletti R., Sampaolesi M., Torgovnick A., Baiocchi M., Padula F., Pisaniello A., Molinaro M., Cossu G., Levrero M., and Bouché M.. Bisperoxovanadium, a phospho-tyrosine phosphatase inhibitor, reprograms myogenic cells to acquire a pluripotent, circulating phenotype. Faseb J., 21: 3573-3583, 2007. Epub 2007 Jun 29.
• D’Andrea M., Pisaniello A., Serra C., Senni M.I., Castaldi L., Molinaro M., Bouché M. Protein kinase C theta co-operates with calcineurin in the activation of slow muscle genes in cultured myogenic cells. J. Cell. Physiol., 207: 379-388, 2006.
• Serra C, Federici M, Buongiorno A, Senni MI, Morelli S, Segratella E, Pascuccio M, Tiveron C, Mattei E, Tatangelo L, Lauro R, Molinaro M, Giaccari A, Bouche M. Transgenic mice with dominant negative PKC-theta in skeletal muscle: a new model of insulin resistance and obesity. J Cell Physiol. 2003; 196:89-97.
• Pisaniello A, Serra C, Rossi D, Vivarelli E, Sorrentino V, Molinaro M, Bouche M. The block of ryanodine receptors selectively inhibits fetal myoblast differentiation. J Cell Sci. 2003; 116:1589-97.
• Mauro A, Ciccarelli C, De Cesaris P, Scoglio A, Bouche M, Molinaro M, Aquino A, Zani BM. PKCalpha-mediated ERK, JNK and p38 activation regulates the myogenic program in human rhabdomyosarcoma cells. J Cell Sci. 2002; 115:3587-99.
• Cossu G, De Angelis L, Borello U, Berarducci B, Buffa V, Sonnino C, Coletta M, Vivarelli E, Bouche M, Lattanzi L, Tosoni D, Di Donna S, Berghella L, Salvatori G, Murphy P, Cusella-De Angelis MG, Molinaro M. Determination, diversification and multipotency of mammalian myogenic cells. Int J Dev Biol. 2000; 44:699-706.
• Bouche M, Canipari R, Melchionna R, Willems D, Senni MI, Molinaro M. TGF-beta autocrine loop regulates cell growth and myogenic differentiation in human rhabdomyosarcoma cells. Faseb J. 2000; 14:1147-58.
• Giorgi, S., Polimeni, M., Senni, M.I., De Gregorio, L., Dragani, T.A., Molinaro, M. and Bouché, M. Isolation and characterization of the murine zinc finger coding gene, ZT2: expression in normal and transformed myogenic cells. Gene, 1999; 230: 81-90.
• Cusella-De Angelis, M.G., Salvatori, G., Zappelli, F., Cossu, G., Molinaro, M. and Bouché, M. Paracrine interactions in the development of skeletal muscle. Basic and Applied Myology, 1996; 6: 397-405.
• Zappelli, F., Willems, D., Osada, S., Ohno, S., Wetsel, W.C., Molinaro, M., Cossu, G. and Bouché, M. The inhibition of differentiation caused by TGF in fetal myoblasts is dependent upon selective expression of PKC. A possible molecular basis for myoblast diversification during limb histogenesis. Dev. Biol., 1996; 180: 156-164.
• Polimeni, M., Giorgi, S., De Gregorio, L., Dragani, T.A., Molinaro, M., Cossu, G., and Bouché, M. Differentiation dependent expression in muscle cells of ZT3, a novel zinc finger factor differentially expressed in embryonic and adult tissues. Mech. Dev. 1996; 54: 1-11.
• Bouché, M., Zappelli, F., Polimeni, M., Adamo, S., Wetsel, W.C., Senni, M.I., and Molinaro, M. Rapid activation- down regulation of PKCα in TPA-induced differentiation of human rhabdomyosarcoma cells. Cell Growth and Diff. (ora Mol. Cancer Res.) 1995; 6:845-852.
• Cusella-De Angelis, M.G., Molinari, S., Le Donne, A., Coletta, M., Vivarelli, E., Bouché, M., Molinaro, M., Ferrari, S., and Cossu, G. Differential response of embryonic and fetal myoblasts to TGFβ: a possible regulatory mechanism of skeletal muscle histogenesis. Development 1994; 120:925-933.
• Bouché, M., Senni, M.I., Grossi, A.M., Zappelli, F., Polimeni, M., Arnold, H.H., Cossu, G., and Molinaro, M. TPA-induced differentiation of human rhabdomyosarcoma cells: expression of the myogenic regulatory factors. Exp. Cell Res. 1993; 208:209-217.
• De Angelis, L., Cusella-De Angelis, M.G., Bouché, M., Vivarelli, C., Boitani, C., Molinaro, M. and Cossu, G. ACTH-like peptides in post-implantation mouse embryos: a possible role in myoblast proliferation and muscle histogenesis. Dev. Biol. 1992; 151, 446-458.
• Cusella De Angelis, M.G., Lyons, G., Sonnino, C.,De Angelis, L., Vivarelli, E., Farmer, K., Wright, W.E., Molinaro, M., Bouché, M., Buckingham, M. and Cossu, G. MyoD, Myogenin independent differentiation of primordial myoblasts in mouse somites. J. Cell Biol. 1992; 116, 1243-1255.
• Cossu, G., M.G. Cusella-De Angelis, L. De Angelis, A. Mezzogiorno, P. Murphy, M. Coletta, E. Vivarelli, M. Bouché, and M. Molinaro. 1992. Multiple myogenic cell precursors and their possible role in muscle histogenesis. In Neuromuscular Development and Disease. A.M. Kelly and H.M. Blau, editors. Raven Press, Ltd., New York. 183–194.
• Aguanno, S., Bouché, M., Adamo, S. and Molinaro, M. TPA induced differentiation of a human rhabdomyosarcoma cell line. Cancer Res. 1990; 50, 3377-3382.
• Cossu, G., Cusella-De Angelis, M.G., Senni, M.I., De Angelis, L., Vivarelli, E., Bouché, M., Boitani, C. and Molinaro, M. Adrenocorticotropin is a specific mitogen for mammalian myogenic cells. Dev. Biol. 1989; 131, 331-336.
• Goldfine, S.M., Peng, I., Bouche, M. and Fischman, D.A. Incorporation of newly synthezised protein by myofibrils and myofilaments in a cell-free system. In "Cellular & Molecular Biology of Muscle Development", Stockdale, F. & Kedes, L. Eds., Alan R. Liss,Inc., New York, N.Y, 1988.
• Bouché, M., Goldfine, S.M. and Fischman, D.A. Posttranslational incorporation of contrattile proteins into myofibrils in a cell-free system. J. Cell Biol. 1988; 107, 587-596.
• Grassi, F., Bouché, M., Aguanno, S., Molinaro, M. and Eusebi, F. Single Acetylcholine-activated channels in Cultured rabdomyoblasts. Exp. Cell Res. 1987; 171, 498-502.
• Bouché, M., Angelini, A., Zani, B.M., Adamo, S. and Molinaro, M. Studies on the mechanism of action of a tumor promoter in cultured cells. J. Exp. Clin. Cancer Res. 1985; 4, 97-105.
• Bouché, M., Adamo, S. and Molinaro, M. Phosphorylation of specific polypeptides induced by 12-O-tetradecanoyl phorbol-13-acetate in chick embryo fibroblests. Carcinogenesis 1984; 5, 559-563,.
• Bouché, M., Adamo, S. and Molinaro, M. Specific TPA induced protein phosphorylation in cultured myotubes. Cell Biol. Int. Rep. 1983; 7, 189.
• Boitani, C., Cossu, G., Bouche, M., Molinaro, M., Pacifici, M. Changes in protein and glycoprotein biosynthesis during differentiation of satellite cells in vitro. Exp. Cell Res. 1982; 138, 489-494.
• Cossu, G., Pacifici, M., Adamo, S., Bouché, M. and Molinaro, M. TPA induced inhibition of the expression of differentiative traits in cultured myotubes: dependence on protein synthesis. Differentiation 1982; 21, 62-65.
• Cossu, G., Zani, B., Coletta, M., Bouché, M., Pacifici, M. and Molinaro, M. In vitro differentiation of satellite cells isolated from normal and dystrophic mammalian muscle. A comparison with embryonic myogenic cells. Cell Differ. 1980; 9, 357-368.
• Cossu, G., Bouché, M., Zani, B., Molinaro M. Differentiation in Culture of Myogenic Cells from Adult Mouse Muscle. Ital. J. Zool. 1978; 45, 369-374

De Felici M, Boitani C, Bouche M, Canipari R, Dolfi A, Filippini a, Musarò A, Papaccio G, Salustri A. Embriologia Umana: morfogenesi, processi molecolari, aspetti clinici. Piccin Ed. 2014. (Textbook in Human Embriology for Medical and Biomedical technology students).