CUTRUZZOLA' FRANCESCA

Professore Ordinario    Vai alla scheda del docente
Settore scientifico disciplinare di riferimento  (BIO/11)
Ateneo Università degli Studi di ROMA "La Sapienza" 
Struttura di afferenza Dipartimento di SCIENZE BIOCHIMICHE "ALESSANDRO ROSSI FANELLI" 
Recapiti Elenco recapiti telefonici
E-Mail francesca.cutruzzola@uniroma1.it
Fax 06-4440062
Web personal website www.macinmec.it

Orari di ricevimento

Lunedì ore 14-16 Monday 2-4 pm

Curriculum

CURRICULUM BREVE Francesca Cutruzzolà si è laureata in Biologia ed ha ottenuto il dottorato in BIochimica presso l'Università di Roma La Sapienza. Nel 1994 ha iniziato la sua carriera accademica come ricercatore e poi nel 2000 come professore associato di Biochimica (BIO/10). Dal 2011 è Professore ordinario di Biologia Molecolare (BIO/11) presso il Dipartimento di Scienze Biochimiche A. Rossi Fanelli della Sapienza. Ha trascorso diversi periodi di studio e ricerca in qualificate istituzioni estere, tra cui:Univ. of Illinois Urbana-Champaign (USA), ETH Politechnic Zurich (CH), Univ. of California-Davis (USA), Univ. of Leiden (NL), Weizmann Institute of Science (IL) and the MRC Toxicology Unit, Leicester (UK) . E' autrice di oltre 100 lavori su riviste internazionali (H-index 28) ed un brevetto. Ha presentato i risultati della ricerca in oltre 30 congressi nazionali ed internazionali. Ha ricevuto finanziamenti da Istituzioni nazionali ed Internazionali (MIUR, CNR, Sapienza Univ., Institute Pasteur-Fondazione Cenci Bolognetti, AIRC, Cystic Fibrosys Foundation, EU). Collabora attivamente con gruppi europei ed extraeuropei. E' revisore esperto per MIUR (ERC LS1-1, LS1-2) ed ANVUR (GEV 05/06) of Italy; ad hoc reviewer per J.Biol.Chem, Biochemistry, ARS, J.Inorg Chem, Curr.Op.Microb., Nature Reviews Microbiology ed altre riviste internazionali. Nel 2012, il gruppo di ricerca della prof. Cutruzzolà è stato finalista negli European Life Science Awards. Dal 2014 è membro di : -Direzione Istituto Pasteur-Fondazione Cenci Bolognetti (Italia). -ERC (European Research Council) Grants Evaluation panels . Dal 2016 è Membro Italiano del Management Committee Cost Action 15126 Mobieu Between Atom and Cell: Integrating Molecular Biophysics Approaches for Biology and Healthcare (2016-2020) L'attività scientifica è focalizzata sulla struttura e funzione di proteine coinvolte in processi patologici, studiate mediante un approccio multidisciplinare (biochimica, biologia strutturale, biologia molecolare e cellulare). In particolare, le linee di ricerca attive comprendono lo studio del ruolo di piccole molecole (nucleotidi) e del metabolismo dell'ossido nitrico nella formazione di biofilm da batteri patogeni e, più recentemente, l'analisi della riprogrammazione metabolica nelle cellule tumorali e nell'invecchiamento. SHORT CV (ENGLISH) Francesca Cutruzzolà obtained her degree in Biology and ph.D. in Biochemistry at the Sapienza University of Rome. Since 1994 she enrolled in the same University as an assistant Professor and in 2000 she became Associate Professor of Biochemistry. From 2011 she is Full Professor of Molecular Biology in the Department of Biochemical Sciences “A.Rossi Fanelli” of Sapienza University. She has spent several periods abroad, for her PhD, post-doc and as a visiting scientist, working at the following institutions: Univ. of Illinois Urbana-Champaign (USA), ETH Politechnic Zurich (CH), Univ. of California-Davis (USA), Univ. of Leiden (NL), Weizmann Institute of Science (IL) and the MRC Toxicology Unit, Leicester (UK) . She is author of more than 100 papers published in peer-reviewed international journals (H-index 28) and one patent; she has been invited to present her work in over 30 national and international meetings. Her research is funded by national (MIUR, CNR, Sapienza Univ., Institute Pasteur-Fondazione Cenci Bolognetti, AIRC, Cystic Fibrosys Foundation) and international agencies (EU). She has ongoing collaborations with european and international groups. She is expert project reviewer for MIUR (ERC LS1-1, LS1-2) and ANVUR (GEV 05/06) of Italy; ad hoc reviewer for J.Biol.Chem, Biochemistry, ARS, J.Inorg Chem, Curr.Op.Microb., Nature Reviews Microbiology and other international journals. In 2012, the research group of prof. Cutruzzolà has been selected as one of the three best european research groups in the European Life Science Awards. From 2014 she is a member of: -Scientific Board of Directors of Institute Pasteur-Fondazione Cenci Bolognetti (Italy). -ERC (European Research Council) Grants Evaluation panels. From 2016 she is Italian Member of the Management Committee Cost Action 15126 Mobieu Between Atom and Cell: Integrating Molecular Biophysics Approaches for Biology and Healthcare (2016-2020) The scientific activity of Francesca Cutruzzolà is focused on the molecular aspects of biological regulation regarding protein structure and function. Proteins are responsible for many tasks of cellular life, including cell shape and inner organization, product synthesis and waste cleanup, routine maintenance and communication. Unbalanced production, malfunction or changes in the stability of individual proteins cause pathologies. The goal of her research is to understand how proteins function, and how they control specific aspects of prokaryotic and eukaryotic cells, using a multidisciplinary approach encompassing Biochemistry, Structural Biology, Molecular and Cell Biology. Her research group is now moving one step ahead, trying to couple basic research to translational studies, in order to identify inhibitors of selected protein targets. The main research lines are focused on small molecules (nucleotides) involved in the formation of bacterial biofilm in chronic infections, on the role of nitric oxide and redox metabolism in bacterial pathogens and, more recently, on the metabolic reprogramming in cancer cells and ageing. Publications (Selected - from 2011) 1. Cutruzzolà F, Giardina G, Marani M, Macone A, Paiardini A, Rinaldo S and Paone A (2017). Glucose metabolism in the progression of prostate cancer. Front. Physiol. 8:97. 2. Paiardini A, Tramonti A, Schirch D, Guiducci G, di Salvo ML, Fiascarelli A, Giorgi A, Maras B, Cutruzzolà F, Contestabile R. (2016) Differential 3-bromopyruvate inhibition of cytosolic and mitochondrial human serine hydroxymethyltransferase isoforms, key enzymes in cancer metabolic reprogramming. Biochim Biophys Acta. 2016 Nov;1864(11):1506-17. 3. Vishnu Pawar S, Messina M, Rinaldo S, Cutruzzolà F, Kaever V, Rampioni G, Leoni L. (2016) Novel genetic tools to tackle c-di-GMP-dependent signaling in Pseudomonas aeruginosa. J Appl Microbiol. 120:205-217. 4. Marani M, Paone A, Fiascarelli A, Macone A, Gargano M, Rinaldo S, Giardina G, Pontecorvi V, Koes DR, McDermott LA, Yang T., Paiardini A., Contestabile R., Cutruzzolà F. (2016) A pyrazolopyran derivative preferentially inhibits the activity of human cytosolic hydroxymethyltransferase and induces cell death in lung cancer cells. Oncotarget, 7(4):4570-83. 5. Cutruzzolà F*, Frankenberg-Dinkel N. (2016) Origin and impact of nitric oxide in Pseudomonas aeruginosa biofilms. J Bacteriol. 198(1):55-65. *corresponding author 6. Fernicola S, Torquati I, Paiardini A, Giardina G, Rampioni G, Messina M, Leoni L, Del Bello F, Petrelli R, Rinaldo S, Cappellacci L, Cutruzzolà F. (2015) Synthesis of triazole-linked analogues of c-di-GMP and their interactions with diguanylate cyclase. J Med Chem. 58:8269-84 7. Fernicola S, Paiardini A, Giardina G, Rampioni G, Leoni L, Cutruzzolà F, Rinaldo S. (2015) In silico discovery and in vitro validation of catechol-containing sulfonohydrazide compounds as potent inhibitors of the diguanylate cyclase PleD. J Bacteriol. 198:147-56. 8. Rinaldo S., Paiardini A., Stelitano V., Brunotti P., Cervoni L., Fernicola S., Protano C., Vitali M., Cutruzzolà F.*, Giardina G. (2015) The structural basis of functional diversification of HD-GYP domain revealed by the Pseudomonas aeruginosa PA4781 protein displaying an unselective bi-metallic binding site. J. Bacteriol. 197:1525-35. *corresponding author 9. Paiardini A., Fiascarelli A., Rinaldo S., Koes D.R., Parroni A., Montini G., Marani M., Paone A., McDermott L.A., Contestabile R., Cutruzzolà F. (2015) Screening and In Vitro Testing of Antifolate Inhibitors of Human Cytosolic Serine Hydroxymethyltransferase. ChemMedChem, 10:490-97. 10. Giardina G., Brunotti, P., Fiascarelli A., Cicalini A., Costa M.G.S., Buckle A.M., Di Salvo ML., Giorgi A., Marani, M., Paone A., Rinaldo S., Paiardini A., Contestabile R., Cutruzzolà F. (2015) How pyridoxal 5’-phosphate differentially regulates human cytosolic and mitochondrial serine hydroxymethyltransferase oligomeric state. FEBS J. 282:1225-41. 11. Paone A, Marani M, Fiascarelli A, Rinaldo S, Giardina G, Contestabile R, Paiardini A, Cutruzzolà F (2014) SHMT1 knockdown induces apoptosis in lung cancer cells by causing uracil misincorporation Cell Death Dis, 5: e1525. 12. Rinaldo S, Giardina G, Cutruzzolà F. (2014) Nitrosylation of c heme in cd1-nitrite reductase is enhanced during catalysis. Biochem Biophys Res Commun. 451:449-54. 13. Cutruzzolà F, Arcovito A, Giardina G, della Longa S, D'Angelo P, Rinaldo S. (2014) Distal-proximal crosstalk in the heme binding pocket of the NO sensor DNR. Biometals 27:763-73. 14. Amelio I., Cutruzzolà F.*, Antonov A., Agostini M., Melino G. (2014) Serine and glycine metabolism in cancer. Trends in Biochem. Sci. 39:191-8. *corresponding author 15. Paiardini A, Aducci P, Cervoni L, Cutruzzolà F, Di Lucente C, Janson G, Pascarella S, Rinaldo S, Visconti S, Camoni L. (2014) The phytotoxin fusicoccin differently regulates 14-3-3 proteins association to mode III targets. IUBMB Life. 66:52-62. 16. Cutruzzolà F, Avigliano L, Candi E. (2014) p73 keeps metabolic control in balance. Cell Cycle 13:179-180. 17. Giardina G, Paiardini A, Fernicola S., Franceschini S., Rinaldo S, Stelitano V, Cutruzzolà F. (2013) Investigating the allosteric regulation of YfiN from Pseudomonas aeruginosa: clues from the structure of the catalytic domain. PLoS One, 8:e81324. 18. Stelitano V, Giardina G, Paiardini A, Castiglione N, Cutruzzolà F, Rinaldo S. (2013) C-di-GMP Hydrolysis by Pseudomonas aeruginosa HD-GYP Phosphodiesterases: Analysis of the Reaction Mechanism and Novel Roles for pGpG. PLoS One, 8:e74920. 19. Urbani A, De Canio M, Palmieri F, Sechi S, Bini L, Castagnola M, Fasano M, Modesti A, Roncada P, Timperio AM, Bonizzi L, Brunori M, Cutruzzolà F, De Pinto V, Di Ilio C, Federici G, Folli F, Foti S, Gelfi C, Lauro D, Lucacchini A, Magni F, Messana I, Pandolfi PP, Papa S, Pucci P, Sacchetta P, The Italian Mt-Hpp Study Group-Italian Proteomics Association Www Itpa It. (2013) The mitochondrial Italian Human Proteome Project initiative (mt-HPP). Mol Biosyst. 9:1984-92. 20. Antoniani D, Rossi E, Rinaldo S, Bocci P, Lolicato M, Paiardini A, Raffaelli N, Cutruzzolà F, Landini P. (2013) The immunosuppressive drug azathioprine inhibits biosynthesis of the bacterial signal molecule cyclic-di-GMP by interfering with intracellular nucleotide pool availability. Appl Microbiol Biotechnol. 97:7325-36. 21. Stelitano, V., Brandt, A., Fernicola, S., Franceschini, S., Giardina, G., Pica, A., Rinaldo, S., Sica, F., Cutruzzolà, F. (2013) Probing the activity of diguanylate cyclases and c-di-GMP phosphodiesterases in real-time by CD spectroscopy. Nucleic Acid Research, 8:e74920. 22. Radoul M., Barak Y., Rinaldo, S., Cutruzzolà, F., Pecht, I., Goldfarb, D. (2012) Solvent Accessibility in the Distal Heme Pocket of the Nitrosyl d1-Heme Complex of Pseudomonas Stutzeri cd1 Nitrite Reductase. Biochemistry, 51:9192-201. 23. Chi, C., Haq, S., Rinaldo, S., Dogan, J., Cutruzzolà, F., Engström, Å., Gianni, S., Lundström, P., Jemth, P. (2012) Interactions outside the boundaries of the canonical binding groove of a PDZ domain influence ligand binding. Biochemistry, 51:8971-9. 24. Rinaldo S., Castiglione N., Giardina G., Caruso M., Arcovito A., Della Longa S., D’Angelo P., Cutruzzolà F. (2012) Unusual heme binding properties of the Dissimilative Nitrate respiration Regulator (DNR), a bacterial nitric oxide sensor. Antioxid Redox Signal. 17:1178-1189 25. Castiglione N., Rinaldo S., Giardina G., Stelitano V., Cutruzzolà F. (2012) Nitrite and Nitrite Reductases: From Molecular Mechanisms to Significance in Human Health and Disease. Antioxid Redox Signal. 17:684-716 26. Giardina G., Montioli R., Gianni S., Cellini, B., Paiardini, A., Borri Voltattorni, C., Cutruzzolà F. (2011) The open conformation of human DOPA decarboxylase reveals the mechanism of PLP addition to Group II decarboxylases. Proc. Natl. Acad. Sci. USA, 108, 20514-20519. 27. Daidone, F., Florio, R., Rinaldo, S., Contestabile, R., di Salvo, M.L., Cutruzzolà, F., Bossa, F., Paiardini, A. (2011) In silico and in vitro validation of serine hydroxymethyltransferase as a chemotherapeutic target of the antifolate drug pemetrexed. Eur J Med Chem. 46,1616-21. 28. Radoul, M., Bykov, D., Rinaldo, S., Cutruzzolà, F., Neese, F., Goldfarb, D. (2011) Dynamic hydrogen bonding network in the distal pocket of the nitrosyl complex of Pseudomonas aeruginosa cd1 nitrite reductase. J. Am. Chem. Soc., 133, 3043-55.. 29. Rinaldo, S., Sam, K.A., Castiglione, N., Stelitano, V., Arcovito, A. Brunori, M., Allen, J.A, Ferguson, S.J., Cutruzzolà, F. (2011) Observation of fast release of NO from ferrous d1 haem allows formulation of a unified reaction mechanism for cytochrome cd1 nitrite reductases. Biochem.J. 435, 217-25.