Catalogo dei Corsi di studio

Education

Secondary: Scientific Lyceum Diploma, final grade 56/60.

University: Biological Sciences Degree at the University of Rome "La Sapienza", July 1981, final grade of 110/110 cum laude. Dissertation on "Studies on the primary structure of the two isoenzymes of aspartate aminotransferase purified from heart horse", supervisor prof. F. Bossa.

Post-lauream courses

May 1982 and March 1983: teorical-pratical courses on high performance liquid cromatografy
October 1982: teorical-pratical course on "Methods in the study of the sequence of proteins", organized by Institute of Organic and Biological Chemistry of the University of Naples
November 1984: course on Genetic Engineering organized by International Institut of Genetic and Biophysics of CNR

Academic Appointments

1983-1986: research fellow from "Farmindustria" on "Purification and structure of biological active peptides"
November1983-February 1984: postdoctoral fellowship at the Department of Biochemistry, Medical College of Virginia, Richmond, VA, USA, for research on purification and characterization of serine hydroxymethyltransferase from E. coli
January 1986-June1986: postdoctoral fellowship at the Department of Biochemistry, Virginia Commonwealth University for research activity on pyridoxal-phosphate dependent enzymes by site-directed mutagenesis techniques
June 1988: research activity at the Department of Biochemistry, Virginia Commonwealth University on mutant forms of E. coli serine hydroxymethyltransferase
1985: permanent position at University of Rome "La Sapienza", Facoltà di Medicina e Chirurgia, as "Funzionario tecnico", VIII grade
2000: permanent position at University of Rome "La Sapienza", Facoltà di Medicina e Chirurgia I, as Assistent Professor/Researcher

Memberships and honours

° Member of Italian Society of Biochemistry

Research interests

° Sebastiana Angelaccio has contributed with different methodologies to the study of structure-function relationship of the pyridoxal phosphate-dependent enzymes such as aspartate aminotransferase (AAT) and serine hydroxymethyltransferase (SHMT). In particular, she has performed a consistent number of site-directed mutagenesis experiments on E. coli SHMT for the identification of amino-acid residues involved in the catalytic mechanism.
Regarding the investigation on Archaea SHMT, she has produced the recombinant form of Methanococcus jannaschii SHMT in E. coli and has carried out experimental studies on the elevated thermostability and thermoactivity of the enzyme and on the binding site of the modified folate used by methanogenes: tetrahydromethanopterin (H4MPT). In addition, she is carring out the production and characterization of appropriate mutant forms of Methanococcus jannaschii SHMT. These site-directed mutagenesis experiments will be helpful to understand the structural basis which confer to this enzyme its tipical features such as solubility and thermostability. Moreover, she performed a structural and functional characterization of SHMT from Psychromonas ingrahanii, an organism adapted to low temperature, recently isolated. The psychrophilic enzyme was produced as recombinant form in Escherichia coli, and subsequently purified and characterized respect to the particular structural and catalytic properties.Her research has also recently focused on the biotechnological exploitation of extremophilic SHMTs in the synthetic production of beta-hydroxy-alpha-amino acids, an important class of natural products that have recently received much attention due to their biological activity on their own and as constituents of many naturally occurring complex compounds, such as antibiotics and immunosupressants. She is also involved in research project on structural and functional characterization on mammalian SHMTs, an attractive target for cancer chemotherapy. Actually, she is involved in a research project regarding the characterization of SHMT from halophytic cyanobacterium Aphanothece halophytica and in the study of the different SHMT isoforms which are present in Arabidopsis thaliana, whose metabolic role is poorly understood.

Teaching duties

- Course of Human Genetics, Corso di Laurea in Infermieristica A, Facoltà of Medicina ed Odontoiatria, Professioni Sanitarie Policlinico Umberto I -
Università La Sapienza, Roma
- Course of Chemistry and Biochemistry, Corso di Laurea in Infermieristica A, Facoltà of Medicina ed Odontoiatria, Professioni Sanitarie Policlinico
Umberto I - Università La Sapienza, Roma
- Course of Chemistry and Biochemistry, Corso di Laurea in Infermieristica E, Facoltà of Farmacia e Medicina, Professioni Sanitarie ASL RM/E, S. Spirito
- Università La Sapienza, Roma
- Course of Clinical Biochemistry, Corso di Laurea in Infermieristica, Facoltà of Medicina e Psicologia, Professioni Sanitarie Azienda S. Camillo Forlanini
- Università La Sapienza, Roma
- Course of Clinical Biochemistry, Corso di Laurea B in Tecnici di Laboratorio Biomedico, Facoltà of Medicina e Odontoiatria and Farmacia e Medicina,
Professioni Sanitarie Azienda S. Camillo Forlanini - Università La Sapienza, Roma

- Responsabile Didattico of the Insegnamento Basi Molecolari e Cellulari della Vita of the Corso di Laurea in Infermieristica A, Facoltà of Medicina ed Odontoiatria, Professioni Sanitarie Policlinico Umberto I - Università La Sapienza, Roma and of the Corso di Laurea in Infermieristica E, Facoltà of Farmacia e Medicina, Professioni Sanitarie ASL RM/E, S. Spirito - Università La Sapienza, Roma
- Supervision of graduate and PhD students.

Scientific Publications

PRIMARY STRUCTURE OF ASPARTATE AMINOTRANSFERASE FROM HORSE HEARTH AND COMPARISON WITH THAT OF OTHER HOMOTOPIC AND HETEROTOPIC ISOENZYMES
F. Martini, S. Angelaccio, D. Barra, S. Doonan and F. Bossa
(1983) Comp. Biochem. Physiol., 73B, 483-487 Cit 2

SEQUENCE HOMOLOGY BETWEEN PROKARYOTIC AND EUKARYOTIC FORMS OF SERINE HYDROXYMETHYLTRANSFERASE
D. Barra, F. Martini, S. Angelaccio, F. Bossa, F. Gavilanes, D. Peterson, B. Bullis and L. Schirch
(1983) Biochem. Biophys. Res. Communication, 116, 1007-1012 Cit 1

PARTIAL AMINO-ACID SEQUENCE AND CYSTEINE REACTIVITIES OF CYTOSOLIC ASPARTATE AMINOTRANSFERASE FROM HORSE HEART
F. Martini, S. Angelaccio, D. Barra, S. Doonan and F. Bossa
(1984) Biochim. Biophys. Acta, 789, 51-56 Cit 0

SERINE HYDROXYMETHYLTRANSFERASE FROM ESCHERICHIA COLI: PURIFICATION AND PROPERTIES
V. Schirch, S. Hopkins, E. Villar and S. Angelaccio
(1985) J. Bacteriol., 163, 1-7 cit 108

THE PRIMARY STRUCTURE OF MITOCHONDRIAL ASPARTATE AMINOTRANSFERASE FROM HUMAN HEART
F. Martini, S. Angelaccio, D. Barra, S. Pascarella, B. Maras, S: Doonan and F. Bossa
(1985) Biochim. Biophys. Acta, 832, 46-51 Cit 23

THE COMPLETE AMINO ACID SEQUENCES OF CYTOSOLIC AND MITOCHONDRIAL ASPARTATE AMINOTRANSFERASE FROM HORSE HEART, AND INFERENCES ON EVOLUTION OF THE ISOENZYMES
S. Doonan, F. Martini, S. Angelaccio, s. Pascarella, D. Barra and F. Bossa
(1986) J. Mol. Evol., 23, 328-335 Cit 29

THE PRIMARY STRUCTURE OF RABBIT LIVER CYTOSOLIC SERINE HYDROXYMETHYLTRANSFERASE
F. Martini. S. Angelaccio, S. Pascarella, D. Barra, F. Bossa and V. Schirch
(1987) J. Biol. Chem., 262, 5499-5509 Cit 47

THE PRIMARY STRUCTURE OF RABBIT LIVER MITOCHONDRIAL SERINE HYDROXYMETHYLTRNSFERASE
F. Martini, B. Maras, P. Tanci, S. Angelaccio, S. Pascarella, D. Barra, F. Bossa and V. Schirch
(1989) J. Biol. Chem., 264, 8509-8519 Cit 37

DETERMINATION OF AMINO ACID REPLACEMENT IN MUTANT FORMS OF SERINE HYDROXYMETHYLTRANSFERASE
D. Barra, S. Angelaccio, M. E. Schininà and F. Bossa
(1990) Journal of Protein Chemistry, 9, 255-256, 13

ASSESSMENT OF SEQUENCE FEATURES IN INTERNAL REGIONS OF PROTEINS
D. Barra, B. Maras, M. E. Schininà, S. Angelaccio and F. Bossa
(1991) Biotechnol. Appl. Biochem., 13, 48-53 cit 7

SERINE HYDROXYMETHYLTRANSFERASE: ORIGIN OF SUBSTRATE SPECIFICITY
S. Angelaccio, S. Pascarella, E. Fattori, F. Bossa, W. Strong and V. Schirch
(1992) Biochemistry, 31, 155-162 Cit 33

FUNCTION OF THE ACTIVE-SITE LYSINE IN ESCHERICHIA COLI SERINE HYDROXYMETHYLTRANSFERASE
d. Schirch, S. Delle fratte, S. Iurescia, S. Angelaccio, R. Contestabile, F. Bossa and V. Schirch
(1993) J. Biol. Chem., 268, 23132-23138 Cit. 45

THE FUCTION OF ARGININE 363 AS THE SUBSTRATE CARBOXYL-BINDING SITE IN ESCHERICHIA COLI SERINE HYDROXYMETHYLTRANSFERASE
S. Delle Fratte, S. Iurescia, S. Angelaccio, F. Bossa and V. Schirch
(1994) Eur. J. Biochem., 225, 395-401 Cit 23

SITE-DIRECTED MUTAGENESIS TECHNIQUES IN THE STUDY OF ESCHERICHIA COLI SERINE HYDROXYMETHYLTRANSFERASE
S. Iurescia, I. Condo , S. Angelaccio, S. Delle Fratte and F. Bossa
(1996) Protein Expr. Purif., 7, 323-328 cit 14

THE STRUCTURE OF SERINE HYDROXYMETHYLTRANSFERASE AS MODELED BY HOMOLOGY AND VALIDATED BY SITE-DIRECTED MUTAGENESIS
S. Pascarella, S. Angelaccio, R. Contestabile,S. Delle Fratte, M. Di Salvo and F. Bossa
(1998) Protein Sci., 7, 1976-1982 Cit 8

THE CONTRIBUTION OF A CONFORMATIONALLY-MOBILE, ACTIVE-SITE LOOP TO THE REACTION CATALYSED BY GLUTAMATE SEMIALDEHYDE AMINOMUTASE
R. Contestabile, S. Angelaccio, F. Bossa and R. A. John
(2000) J. Biol. Chem, 275, 3879-3886 Cit 13

ROLE OF TYROSINE 65 IN THE MECHANISM OF SERINE HYDROXYMETHYLTRANSFERASE
R. Contestabile, S. Angelaccio, F. Bossa, H. T. Wright, N. Scardale, G. Kazanina and V. Schirch
(2000) Biochemistry, 39, 7492-7500 Cit 15

SITE-DIRECTED MUTAGENESIS BY THE MEGAPRIMER PCR METHOD: VARIATIONS ON A THEME FOR SIMULTANEOUS INTRODUCTION OF MULTIPLE MUTATIONS
S. Angelaccio and M. C. Bonaccorsi di Patti
(2002) Anal. Biochem., 306, 346-349 Cit 17

CATALYTIC AND THERMODYNAMIC PROPERTIES OF TETRAHYDROMETHANOPTERIN-DEPENDENT SERINE HYDROXYMETHYLTRANSFERASE FROM METHANOCOCCUS JANNASCHII
S. Angelaccio, R. Chiaraluce, V. Consalvi, B. Buchenau, L. Giangiacomo, F. Bossa and R. Contestabile
(2003) J. Biol. Chem., 278, 41789-41797 Cit 14

CRYSTALIZATION AND PRELIMINARY X-RAY CRYSTALLOGRAPHIC ANALYSIS OF THE LAMINARINASE ENDO-BETA-1,3-GLUCANASE FROM PYROCOCCUS FURIOSUS.
Ilari A, Angelaccio S, Fiorillo A, Florio R, Consalvi V, Chiaraluce R.
(2004) Acta Crystallogr D Biol Crystallogr., 60(Pt 12 Pt 2), 2394-5 Cit 1

CALCIUM-INDUCED TERTIARY STRUCTURE MODIFICATIONS OF ENDO-BETA-1,3-GLUCANASE FROM PYROCOCCUS FURIOSUS IN 7.9 M GUANIDINIUM CHLORIDE.
Chiaraluce R, Gianese G, Angelaccio S, Florio R, van Lieshout JF, van der Oost J, Consalvi V.
(2005) Biochem J., 386 (Pt 3), 515-524 Cit 5

TERTIARY STRUCTURE IN 7.9 M GUANIDINIUM CHLORIDE THE ROLE OF GLU 53 AND ASP287 IN PYROCOCCUS FURIOSUS ENDO-BETA-1,3-GLUCANASE
Chiaraluce R., Florio R., Angelaccio S., Gianese G., van Lieshout JF, van der Oost J. and Consalvi V.
(2007) The Febs Journal, 274, 6167-6179. Cit 1

CRYSTAL STRUCTURE OF A FAMILY 16 ENDOGLUCANASE FROM THE HYPERTHERMOPHILE PYROCOCCUS FURIOSUS-STRUCTURAL BASIS OF SUBSTRATE RECOGNITION
Ilari A, Fiorillo A, Angelaccio S, Florio R, Chiaraluce R, van der Oost J, Consalvi V.
(2009) FEBS J. , 276(4):1048-58. Epub 2008 Jan 16. Cit. 7

ROLE OF A CONSERVED ACTIVE SITE CATION-PI INTERACTION IN ESCHERICHIA COLI SERINE HYDROXYMETHYLTRANSFERASE
Vivoli M, Angelucci F, Ilari A, Morea V, Angelaccio S, di Salvo ML, Contestabile R.
(2009) Biochemistry. Dec 22;48(50):12034-46. Cit. 6

SERINE HYDROXYMETHYLTRANSFERASE FROM THE COLD-ADAPTED MICRORGANISM PSYCHROMONAS INGRAHAMII: A LOW TEMPERATURE ACTIVE ENZYME WITH BROAD SUBSTRATE SPECIFICITY
Angelaccio S., Florio R., Consalvi V., Festa G., Pascarella S.
(2012) Int. J. Mol. Sci., 13: 1314-1326. Cit. 2

EXTREMOPHILIC SHMTs: FROM STRUCTURE TO BIOTECHNOLOGY
Sebastiana Angelaccio
(2013) BioMed Research International, vol. 2013, Article ID 851428, 10 pages, doi: 10.1155/2013/851428

CONFORMATIONAL TRANSITIONS DRIVEN BY PYRIDOXAL-5 PHOSPHATE UPTAKE IN THE PSYCHROPHILIC SERINE HYDROXYMETHYLTRANSFERASE FROM PSYCHROMONAS INGRAHAMII
(2014) Angelaccio S, Dworkowski F, Di Bello A, Milano T, Capitani G, Pascarella S.
Proteins. 2014 Oct;82(10):2831-41. doi: 10.1002/prot.24646. Epub 2014 Aug 5.

THE CRYSTAL STRUCTURE OF ARCHAEAL SERINE HYDROXYMETHYLTRANSFERASE REVEALS IDIOSYNCRATIC FEATURES LIKELY REQUIRED TO WITHSTAND HIGH TEMPERATURES
Angelucci F, Morea V, Angelaccio S, Saccoccia F, Contestabile R, Ilari A.
Proteins. 2014 Dec;82(12): 3437-49. doi: 10.1002/prot.24697.

STRUCTURAL STABILITY OF COLD-ADAPTED SERINE HYDROXYMETHYLTRANSFERASE, A TOOL FOR ß-HYDROXY- -AMINO ACID BIOSYNTHESIS
Sebastiana Angelaccio, Martino Luigi di Salvo, Alessia Parroni, Angela Di Bello, Roberto Contestabile, Stefano Pascarella
Journal of Molecular Catalysis. B, Enzymatic. 2014, 110; 171-177 DOI information: 10.1016/j.molcatb.2014.10.007

CONSERVED WATER MOLECULES IN BACTERIAL SERINE HYDROXYMETHYLTRANSFERASE
Teresa Milano; Martino Luigi Di Salvo; Sebastiana Angelaccio; Stefano Pascarella
(2015) Protein Engineering Design and Selection
Doi: 10.1093/protein/gzv026

A BIOINFORMATICS ANALYSIS REVEALS A GROUP OF MocR BACTERIAL TRANSCRIPTIONAL REGULATORS LINKED TO A FAMILY OF GENES CODING FOR MEMBRANE PROTEINS
Milano T, Angelaccio S, Tramonti A, di Salvo ML, Contestabile R, Pascarella S.
Biochemistry Research International, vol. 2016, Article ID 4360285, 13 pages, 2016. doi:10.1155/2016/4360285

STRUCTURAL PROPERTIES OF THE LINKERS CONNECTING THE N- AND C-TERMINAL DOMAINS IN THE MocR BACTERIAL TRANSCRIPTIONAL REGULATORS
Milano T, Angelaccio S, Tramonti A, di Salvo ML, Contestabile R, Pascarella S.
Biochemie Open, Volume 3, December 2016, pp 8-18

DATA FROM COMPUTATIONAL ANALYSIS OF THE PEPTIDE LINKERS IN THE MocR BECTERIAL TRANSCRIPTIONAL REGULATORS
Angelaccio S, Milano T, , Tramonti A, di Salvo ML, Contestabile R, Pascarella S.
Biochemie Open, Volume 9, December 2016, pp 292-313.

A COMPREHENSIVE COMPUTATIONAL ANALYSIS OF MYCOBACTERIUM GENOMES PINPOINTS THE GENES CO-OCCURRING WITH YczE, A MEMBRANE PROTEIN CODING GENE UNDER THE PUTATIVE CONTROL OF A MocR, AND PREDICTS ITS FUNCTION
Milano T, Angelaccio S, Tramonti A, di Salvo ML, Nogues I, Contestabile R, Pascarella S.
Interdiscip Sci. 2018 Mar;10(1):111-125. doi: 10.1007/s12539-017-0266-z. Epub 2017 Nov 2.

STRUCTURAL BASIS OF METHOTEXATE AND PEMETREXED ACTION ON SERINE HYDROXYMETHYLTRANSFERASE REVEALED USING PLANT MODEL
Ruszkowski, M., Sekula, B., Ruszkowska, A., Contestabile, R., Nogues, I., Angelaccio, S., Szczepaniak, A., Dauter, Z.
Sci Rep 9, 19614 (2019) doi:10.1038/s41598-019-56043-4
STRUCTURAL AND KINETIC PROPERTIES OF SERINE HYDROXYMETHYLTRANSFERASE FROM THE HALOPHYTIC CYANOBACTERIUM APHANOTHECE HALOPHYTICA PROVIDE A RATIONALE FOR SALT TOLERANCE
Isabel Nogués, Angela Tramonti, Sebastiana Angelaccio, Milosz Ruszkowski, Bartosz Sekula, Roberto Contestabile
International Journal of Biological Macromolecules 159 (2020) 517-529

ARABIDOPSIS THALIANA SERINE HYDROXYMETHYLTRANSFERASE: FUNCTIONS, STRUCTURES AND PERSPECTIVES
Isabel Nogués, Bartosz Sekula, Sebastiana Angelaccio, Marta Grzechowiak, Angela Tramonti, Roberto Contestabile, Milosz Ruszkowski
Plant Physiology and Biochemistry 187 (2022) 37-49

INSIGHTS INTO THE SUBSTRATE SPECIFICITY, STRUCTURE, AND DYNAMICS OF PLANT HISTIDINOL-PHOSPHATE AMINOTRANSFERASE (HISN6)
Maria Rutkiewicz, Isabel Nogues, Wojciech Witek, Sebastiana Angelaccio, Roberto Contestabile, Milosz Ruszkowski
Plant Physiology and Biochemistry 196 (2023) 759-773