Docente
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DI LEONARDO ROBERTO
(programma)
## Part I: What's inside: genetic parts and circuits
1) Course overview, what is biophysics?, *Escherichia coli* as the "hydrogen atom" for biology.
2) The central dogma: DNA-RNA-protein.
3) Regulation of gene expression: the synthetic biology viewpoint.
4) Noise in gene expression
5) Single molecule techniques: fluorescence microscopy, optical tweezers
6) Construction and analysis of basic genetic circuits: toggle switch, repressilator.
7) Genetic editing: delete, insert, upload.
8) Tutorial: howto make bacteria that glow in the dark.
## Part II: What's outside: single cell movements
1) Low Reynolds number hydrodynamics: basic formalism and fundamental theorems.
2) Prokaryotic flagella: structure and dynamics, slender body
3) Eukaryotic flagella: structure, squirmers, synchronization
4) Cytoskeleton: parts and dynamics
5) Interactions: cell-substrate, cell-cell
6) Probing cell dynamics: digital video microscopy, Fourier microscopy, holographic microscopy.
## Part III: Multicellular dynamics
1) Cell growth and division
2) Lab techniques: microfluidics, mother machine
3) Reaction diffusion equations: Fisher-Kolmogorov, waves, branches, fractals
4) Tissues: dynamics of cell monolayers, vertex models, self propelled Voronoi
5) Quorum sensing: engineering morphogenesis with synthetic biology
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